1NC1
Crystal structure of E. coli MTA/AdoHcy nucleosidase complexed with 5'-methylthiotubercidin (MTH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.82650 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.300, 69.300, 127.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.580 - 2.000 |
| Rwork | 0.190 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nc3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | |
| Number of reflections | 30742 | |
| Completeness [%] | 97.8 | 98.9 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | PEG 200, sodium acetate, sodium chloride, cobalt chloride, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
