1NB3
Crystal structure of stefin A in complex with cathepsin H: N-terminal residues of inhibitors can adapt to the active sites of endo-and exopeptidases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 2001-01-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.584, 91.630, 161.235 |
Unit cell angles | 90.00, 93.69, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.161 * | 0.583 * |
Total number of observations | 313477 * | |
Number of reflections | 35154 * | |
<I/σ(I)> | 4.7 | |
Completeness [%] | 97.4 * | 97.1 * |
Redundancy | 8.9 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.2 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 18 (%(w/v)) | |
2 | 1 | reservoir | ammonium sulfate | 0.18 (M) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.2 |
4 | 1 | drop | protein | 7 (mg/ml) | |
5 | 1 | drop | 40 (mM) |