1N8S
Structure of the pancreatic lipase-colipase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991-12-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.300, 80.300, 251.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.040 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.29000 |
| Structure solution method | MIR |
| RMSD bond length | 0.003 |
| RMSD bond angle | 3.500 |
| Data reduction software | AUTOMAR |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.150 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.085 | |
| Total number of observations | 48860 * | |
| Number of reflections | 17720 | |
| Completeness [%] | 92.0 | 62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 293 | NaCl-0.2-0.5M, PEG 8000 2%, MES 0.1M, BetaOG beyond CMC, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | unknown * | 6 | 293 | NaCl-0.2-0.5M, PEG 8000 2%, MES 0.1M, BetaOG beyond CMC, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 0.2-0.5 (M) | ||
| 2 | 1 | 1 | PEG8000 | 2 (%) | |
| 3 | 1 | 1 | MES | 0.1 (M) | pH6.0 |
| 4 | 1 | 1 | HPL | 8 (mg/ml) | |
| 5 | 1 | 1 | PCL | 2.5 (mg/ml) |
