1N7P
Streptococcus pneumoniae Hyaluronate Lyase W292A/F343V Double Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.979, 103.721, 100.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.550 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1loh |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.410 * |
| Data reduction software | MADNESS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.062 | 0.670 |
| Total number of observations | 723619 * | |
| Number of reflections | 127336 * | |
| Completeness [%] | 99.5 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | AMMONIUM SULFATE, SODIUM CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.1 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 60-65 (%sat) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | dioxane | 2 (%) | |
| 5 | 1 | reservoir | sodium citrate | 50 (mM) | pH6.0 |
