1N7P

Streptococcus pneumoniae Hyaluronate Lyase W292A/F343V Double Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	83.979, 103.721, 100.517
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 1.550
R-factor	0.19
Rwork	0.190
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1loh
RMSD bond length	0.014 *
RMSD bond angle	1.410 *
Data reduction software	MADNESS
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.062	0.670
Total number of observations	723619 *
Number of reflections	127336 *
Completeness [%]	99.5	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	AMMONIUM SULFATE, SODIUM CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.1 (mg/ml)
2	1	reservoir	ammonium sulfate	60-65 (%sat)
3	1	reservoir		0.2 (M)
4	1	reservoir	dioxane	2 (%)
5	1	reservoir	sodium citrate	50 (mM)	pH6.0