1N6P
Crystal Structure of Human Rab5a A30E mutant complex with GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.550, 63.670, 65.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.540 |
| R-factor | 0.19 |
| Rwork | 0.184 |
| R-free | 0.20400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.046 | 0.260 |
| Total number of observations | 125870 * | |
| Number of reflections | 22532 * | |
| <I/σ(I)> | 37.6 | 6.6 |
| Completeness [%] | 98.9 * | 98.3 * |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | PEG 6000, sodium chloride, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | GppNHp | 5 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | MES | 50-100 (mM) | pH6.0 |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | 1 (mM) | ||
| 8 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%(v/v)) |
