1N5O
Structural consequences of a cancer-causing BRCA1-BRCT missense mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-08 |
| Detector | SBC-2 |
| Wavelength(s) | 1.0093 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 114.535, 114.535, 121.527 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.27538 |
| Rwork | 0.274 |
| R-free | 0.29800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jnx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.857 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.800 | |
| Rmerge | 0.068 * | 0.404 * |
| Total number of observations | 100697 * | |
| Number of reflections | 12024 | |
| Completeness [%] | 99.3 * | 100 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | ammonium sulphate, cobalt chloride, MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 3 | 1 | reservoir | MES | 100 (mM) | pH6.7 |
| 4 | 1 | reservoir | 10 (mM) |
