1N59

Crystal structure of the Murine class I Major Histocompatibility Complex of H-2KB, B2-Microglobulin, and A 9-Residue immunodominant peptide epitope gp33 derived from LCMV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-06-03
Detector	MARRESEARCH
Wavelength(s)	1.018
Spacegroup name	P 1 21 1
Unit cell lengths	50.551, 88.574, 120.090
Unit cell angles	90.00, 93.72, 90.00

Refinement procedure

Resolution	20.000 * - 2.950
R-factor	0.23195
Rwork	0.229
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1osz
RMSD bond length	0.018
RMSD bond angle	1.890 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.950
High resolution limit [Å]	2.950 *	2.950 *
Rmerge	0.130 *	0.242 *
Total number of observations	46919 *
Number of reflections	20422 *
<I/σ(I)>	8.4	3.5
Completeness [%]	86.8 *	62.2
Redundancy	2.3	2.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG8000, magnesium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	1.85 (M)
2	1	reservoir	MPD	1 (%)
3	1	drop	protein	5 (mg/ml)
4	1	drop	Tris-Cl	20 (mM)	pH7.0