1OSZ
MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN
Summary for 1OSZ
| Entry DOI | 10.2210/pdb1osz/pdb |
| Descriptor | MHC CLASS I H-2KB HEAVY CHAIN, BETA-2 MICROGLOBULIN, VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN (3 entities in total) |
| Functional Keywords | mhc-peptide complex, transmembrane protein, thymic selection, complex (mhc i-peptide), complex (mhc i-peptide) complex, complex (mhc i/peptide) |
| Biological source | Mus musculus (house mouse) More |
| Cellular location | Membrane; Single-pass type I membrane protein: P01901 Secreted: P01887 |
| Total number of polymer chains | 3 |
| Total formula weight | 44322.79 |
| Authors | Ghendler, Y.,Teng, M.-K.,Liu, J.-H.,Witte, T.,Liu, J.,Kim, K.S.,Kern, P.,Chang, H.-C.,Wang, J.-H.,Reinherz, E.L. (deposition date: 1998-06-18, release date: 1999-01-13, Last modification date: 2024-10-09) |
| Primary citation | Ghendler, Y.,Teng, M.K.,Liu, J.H.,Witte, T.,Liu, J.,Kim, K.S.,Kern, P.,Chang, H.C.,Wang, J.H.,Reinherz, E.L. Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands. Proc.Natl.Acad.Sci.USA, 95:10061-10066, 1998 Cited by PubMed Abstract: The T lineage repertoire is shaped by T cell receptor (TCR)-dependent positive and negative thymic selection processes. Using TCR-transgenic (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a single weak agonist peptide variant V4L (L4) inducing phenotypic and functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined and refined to 2.1 A for comparison with the VSV8/Kb structure at similar resolution. Aside from changes on the p4 side chain of L4 and the resulting alteration of the exposed Kb Lys-66 side chain, these two structures are essentially identical. Hence, a given TCR recognizes subtle distinctions between highly related ligands, resulting in dramatically different selection outcomes. Based on these finding and the recent structural elucidation of the N15-VSV8/Kb complex, moreover, it appears that the germ-line Valpha repertoire contributes in a significant way to positive selection. PubMed: 9707600DOI: 10.1073/pnas.95.17.10061 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
Download full validation report
