1N56
Y-family DNA polymerase Dpo4 in complex with DNA containing abasic lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200HB |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-15 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.016, 102.358, 105.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.400 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jx4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.270 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.900 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.038 | 0.399 |
Number of reflections | 39503 | |
Completeness [%] | 93.5 | 80 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG 3350, 0.1M Hepes, 100mM calcium acetate, 2.5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | Hepes | ||
3 | 1 | 1 | calcium acetate | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 2 | PEG 3350 | ||
6 | 1 | 2 | glycerol | ||
7 | 1 | 2 | calcium acetate | ||
8 | 1 | 2 | Hepes |