1N52

Cap Binding Complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	200
Detector technology	CCD
Collection date	2002-01-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	111.681, 111.681, 177.588
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 * - 2.120 *
Rwork	0.223
R-free	0.24270 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6k
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.170
High resolution limit [Å]	2.120	2.110
Rmerge	0.058 *
Number of reflections	78602 *
<I/σ(I)>	16	2.4
Completeness [%]	99.0 *
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	4 *	PEG 400, magnesium chloride, Tris, Glycine, Ethylene Glycol , pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.25	4 *	PEG 400, magnesium chloride, Tris, Glycine, Ethylene Glycol , pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG400	22 (%(w/v))
3	1	reservoir	Tris-HCl	100 (mM)	pH7.25
4	1	reservoir		125 (mM)
5	1	reservoir	glycerol	10 (%(v/v))
6	1	reservoir	dithiothreitol	2 (mM)
7	1	reservoir	glycine	10 (mM)
8	1	reservoir	ethylene glycol	3 (%(v/v))