1N4U
CHOLESTEROL OXIDASE FROM STREPTOMYCES @ pH 4.5 (STREPTOMYCES SP. SA-COO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.248, 73.276, 63.098 |
| Unit cell angles | 90.00, 105.07, 90.00 |
Refinement procedure
| Resolution | 46.900 - 0.950 |
| Rwork | 0.113 |
| R-free | 0.13580 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | 1mxt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.058 | 0.570 |
| Number of reflections | 266037 | |
| <I/σ(I)> | 11.1 | 1.49 |
| Completeness [%] | 94.3 | 89.3 |
| Redundancy | 3.9 | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.5 |
