1N4S
Protein Geranylgeranyltransferase type-I Complexed with GGPP and a Geranylgeranylated KKKSKTKCVIL Peptide Product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 271.121, 268.426, 184.818 |
| Unit cell angles | 90.00, 131.58, 90.00 |
Refinement procedure
| Resolution | 29.980 - 2.600 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.069 * | 0.435 * |
| Total number of observations | 1011902 * | |
| Number of reflections | 300000 | |
| <I/σ(I)> | 13.6 | 2.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 17 * | ammonium sulfate, sodium citrate, dithiothreitol , pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.3 (M) | |
| 3 | 1 | reservoir | 175 (mM) | pH6.5 | |
| 4 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 5 | 1 | reservoir | MES | 100 (mM) | pH6.3 |
