1N4R
Protein Geranylgeranyltransferase type-I Complexed with a Geranylgeranylated KKKSKTKCVIL Peptide Product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00008 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 272.068, 268.801, 185.310 |
| Unit cell angles | 90.00, 131.55, 90.00 |
Refinement procedure
| Resolution | 29.860 - 2.800 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.21700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.180 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 * | 0.432 * |
| Total number of observations | 785048 * | |
| Number of reflections | 240519 * | |
| <I/σ(I)> | 13 | 2.3 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 17 * | ammonium sulfate, sodium citrate, dithiothreitol , pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.3 (M) | |
| 3 | 1 | reservoir | 175 (mM) | pH6.5 | |
| 4 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 5 | 1 | reservoir | MES | 100 (mM) | pH6.3 |
