1N4P

Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.900000
Spacegroup name	C 1 2 1
Unit cell lengths	272.338, 271.566, 185.425
Unit cell angles	90.00, 131.56, 90.00

Refinement procedure

Resolution	29.920 - 2.650
R-factor	0.204
Rwork	0.204
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.180 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.740
High resolution limit [Å]	2.650	2.650
Rmerge	0.077 *	0.500 *
Total number of observations	1038571 *
Number of reflections	286863
<I/σ(I)>	14	2.2
Completeness [%]	98.7	91.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	17 *	ammonium sulfate, sodium citrate, dithiothreitol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	1.3 (M)
3	1	reservoir		175 (mM)	pH6.5
4	1	reservoir	dithiothreitol	20 (mM)
5	1	reservoir	MES	100 (mM)	pH6.3