1N4P
Protein Geranylgeranyltransferase type-I Complexed with Geranylgeranyl Diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 272.338, 271.566, 185.425 |
| Unit cell angles | 90.00, 131.56, 90.00 |
Refinement procedure
| Resolution | 29.920 - 2.650 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.180 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.077 * | 0.500 * |
| Total number of observations | 1038571 * | |
| Number of reflections | 286863 | |
| <I/σ(I)> | 14 | 2.2 |
| Completeness [%] | 98.7 | 91.5 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 17 * | ammonium sulfate, sodium citrate, dithiothreitol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.3 (M) | |
| 3 | 1 | reservoir | 175 (mM) | pH6.5 | |
| 4 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 5 | 1 | reservoir | MES | 100 (mM) | pH6.3 |
