1N3S

Biosynthesis of pteridins. Reaction mechanism of GTP cyclohydrolase I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-08
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 43 21 2
Unit cell lengths	124.302, 124.302, 389.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.550
R-factor	0.271
Rwork	0.262
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.940
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.550
Number of reflections	76546
Completeness [%]	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	Peg6000, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K