1N3L
Crystal structure of a human aminoacyl-tRNA synthetase cytokine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.595, 162.392, 35.603 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.180 |
R-factor | 0.1802 |
Rwork | 0.180 |
R-free | 0.21590 * |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 2.500 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.220 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.054 | 0.699 |
Number of reflections | 134129 | |
<I/σ(I)> | 55.2 | 1.004 |
Completeness [%] | 93.0 * | 59 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.38 | 279 | (NH4)2SO4, NaH2PO4, K2HPO4, acetone, pH 6.38, VAPOR DIFFUSION, SITTING DROP, temperature 279K |