1N26

Crystal Structure of the extra-cellular domains of Human Interleukin-6 Receptor alpha chain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	51.130, 51.130, 303.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.22
Rwork	0.220
R-free	0.29000
Structure solution method	MIR
Starting model (for MR)	ab initio built into MIR map
RMSD bond length	0.015
RMSD bond angle	1.960
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.400	2.350
Rmerge	0.110	0.570
<I/σ(I)>	39	3.7
Completeness [%]	99.4	100
Redundancy	18.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	300	ammonium sulphate, PEG 3350, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	8 *	300	ammonium sulphate, PEG 3350, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	8 *	300	ammonium sulphate, PEG 3350, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	8 *	300	ammonium sulphate, PEG 3350, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)	pH8.0
3	1	reservoir	ammonium sulfate	50 (mM)
4	1	reservoir	PEG3350	18 (%)
5	1	reservoir	sodium citrate	2.5 (mM)	pH5.6