1N0V
Crystal structure of elongation factor 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.038 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.835, 114.546, 177.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.850 |
| R-factor | 0.233 |
| Rwork | 0.232 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Structure of eEF2-sordarin complex |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.420 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.054 | 0.316 |
| Number of reflections | 45917 | |
| <I/σ(I)> | 39.1 | |
| Completeness [%] | 96.3 | 97 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.2 | 277 * | Joergensen, R., (2002) Acta Crystallogr., Sect.D, 58, 712. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES-NaOH | 20 (mM) | pH7.2 |
| 10 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 11 | 1 | reservoir | 0.9 (mM) | ||
| 12 | 1 | reservoir | PEG8000 | 7-9 (%) | |
| 2 | 1 | drop | 75 (mM) | ||
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 6 | 1 | drop | GDP | 1 (mM) | |
| 7 | 1 | drop | protein | 4.5 (mg/ml) | |
| 8 | 1 | reservoir | HEPES | 0.1 (M) | pH7.2 |
| 9 | 1 | reservoir | ethylene glycol | 5 (%) |
