1MY4
crystal structure of glutamate receptor ligand-binding core in complex with iodo-willardiine in the Zn crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.840, 163.800, 48.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.900 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ftm |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.489 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.282 |
| Number of reflections | 68374 | |
| Completeness [%] | 95.2 * | 79.2 |
| Redundancy | 4.60 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG4K, ammonium sulfate, zinc acetate and Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG4000 | 10-15 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.25-0.3 (M) | |
| 7 | 1 | reservoir | zinc acetate | 0.1-0.16 (M) | |
| 8 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.5 |
