1MY4

crystal structure of glutamate receptor ligand-binding core in complex with iodo-willardiine in the Zn crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9879
Spacegroup name	P 21 21 2
Unit cell lengths	113.840, 163.800, 48.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.900
R-factor	0.213
Rwork	0.213
R-free	0.26200
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ftm
RMSD bond length	0.010 *
RMSD bond angle	1.489 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.053	0.282
Number of reflections	68374
Completeness [%]	95.2 *	79.2
Redundancy	4.60 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	4 *	PEG4K, ammonium sulfate, zinc acetate and Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)	pH7.0
2	1	drop		20 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	reservoir	PEG4000	10-15 (%)
6	1	reservoir	ammonium sulfate	0.25-0.3 (M)
7	1	reservoir	zinc acetate	0.1-0.16 (M)
8	1	reservoir	Tris-HCl	0.1 (M)	pH7.5