1MY3
crystal structure of glutamate receptor ligand-binding core in complex with bromo-willardiine in the Zn crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.061, 163.406, 47.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.750 |
| Rwork | 0.211 |
| R-free | 0.25500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ftm |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.479 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.051 | 0.174 |
| Number of reflections | 86531 | |
| Completeness [%] | 94.4 | 70.5 |
| Redundancy | 5.76 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG8000, ammonium sulfate, zinc acetate and sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 12-16 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.25-0.3 (M) | |
| 7 | 1 | reservoir | zinc acetate | 0.05 (M) | |
| 8 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
