1MWH
REOVIRUS POLYMERASE LAMBDA3 BOUND TO MRNA CAP ANALOG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.891, 84.944, 249.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.500 |
| R-factor | 0.243 |
| Rwork | 0.209 |
| R-free | 0.25200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | reovirus polymerase 1MUK |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.719 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.590 |
| High resolution limit [Å] | 2.500 * | 2.500 |
| Rmerge | 0.088 * | 0.404 * |
| Number of reflections | 49606 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 93.3 * | 80.8 * |
| Redundancy | 5-6 * | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 22 * | PEG4000, sodium chloride, HEPES, glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.7 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 50 (mM) | pH7.8 |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | 150 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 10 (mM) | |
| 6 | 1 | drop | 0.02 (%) | ||
| 7 | 1 | reservoir | PEG8000 | 7.5 (%) | |
| 8 | 1 | reservoir | glycerol | 10 (%) | |
| 9 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.8 |
