1MVL
PPC decarboxylase mutant C175S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-02-26 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 63 |
Unit cell lengths | 111.790, 111.790, 32.996 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.1702 |
Rwork | 0.169 |
R-free | 0.20300 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1e20 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.530 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.220 |
Number of reflections | 15995 | |
Completeness [%] | 98.2 | 98.4 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 * | 293 | ammonium sulphate, imidazole, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
3 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
4 | 1 | reservoir | imidazole-HCl | 100 (mM) | pH6.3 |