1MV8
1.55 A crystal structure of a ternary complex of GDP-mannose dehydrogenase from Psuedomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-08 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 |
| Unit cell lengths | 82.472, 82.472, 309.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.550 |
| R-factor | 0.175 * |
| Rwork | 0.174 |
| R-free | 0.19400 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1muu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.123 | 0.391 |
| Total number of observations | 1557003 * | |
| Number of reflections | 294277 | |
| <I/σ(I)> | 9.1 | 2.1 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 8%v/v methyl propane diol, 0.1M Na Acetate, 1mM DTT, pH 4.6, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
| 2 | 1 | drop | dithiothreitol | 1.0 (mM) | |
| 3 | 1 | drop | protein | 7.5 (mg/ml) | |
| 4 | 1 | reservoir | MPD | 8. (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
