1MT9
Viability of a drug-resistant HIV-1 protease mutant: structural insights for better antiviral therapy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.265, 59.133, 61.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.840 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.21000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mtr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 26.000 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.840 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.330 |
| Total number of observations | 98506 * | |
| Number of reflections | 12659 | 1182 * |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 95.4 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | sodium phosphate, sodium citrate, ammonium sulphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | reservoir | phosphate | 126 (mM) | pH6.2 |
| 3 | 1 | reservoir | sodium citrate | 63 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 28-31 (%) |
