1MT6
Structure of histone H3 K4-specific methyltransferase SET7/9 with AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97780 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.347, 76.038, 166.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.640 - 2.200 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.041 * |
| RMSD bond angle | 3.300 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 * | |
| Number of reflections | 21496 | 1768 * |
| Completeness [%] | 93.0 | 77.1 |
| Redundancy | 3.6 * | 2.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 10 * | PEG 4000, sodium citrate, Isopropanol, DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | imidazole | 20 (mM) | pH7.0 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 6 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 7 | 1 | reservoir | isopropanol | 20 (%(v/v)) |
