1MT5
CRYSTAL STRUCTURE OF FATTY ACID AMIDE HYDROLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 147.107, 272.019, 147.216 |
| Unit cell angles | 90.00, 115.21, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.800 |
| R-factor | 0.22062 |
| Rwork | 0.218 |
| R-free | 0.23700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FAAH structure in an alternate spacegroup. Solved by MAD/MIRAS phasing. |
| RMSD bond length | 0.029 * |
| RMSD bond angle | 1.041 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.800 | |
| Rmerge | 0.104 * | 0.264 * |
| Number of reflections | 173615 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 76.0 * | 79 * |
| Redundancy | 2.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 6000, 2,4-methyl pentanediol, sodium citrate, lithium sulphate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | pH5. |
| 3 | 1 | reservoir | lithium sulfate | 100 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 7 (%(w/v)) | |
| 5 | 1 | reservoir | MPD | 5 (%(v/v)) | |
| 6 | 1 | reservoir | dithiothreitol | 0-20 (mM) |
