1MSS

LARGE SCALE STRUCTURAL REARRANGEMENTS OF THE FRONT LOOPS IN MONOMERISED TRIOSEPHOSPHATE ISOMERASE, AS DEDUCED FROM THE COMPARISON OF THE STRUCTURAL PROPERTIES OF MONOTIM AND ITS POINT MUTATION VARIANT MONOSS

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	46.570, 46.580, 66.650
Unit cell angles	94.47, 69.82, 75.83

Refinement procedure

Resolution	24.000 - 2.400
R-factor	0.198
Rwork	0.198
RMSD bond length	0.010
RMSD bond angle	0.800
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.044 *
Total number of observations	22787 *
Number of reflections	13847 *
Completeness [%]	71.0 *	20 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	100 (mM)
2	1	drop	dithiothreitol	1 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop		1 (mM)
5	1	drop	MPD	5 (%)
6	1	reservoir	PEG8000	10 (%)