1MSC

CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER

Experimental procedure

Collection date	1993-12-07
Spacegroup name	P 21 21 2
Unit cell lengths	76.200, 55.700, 28.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.2
RMSD bond length	0.021
RMSD bond angle	0.066
Data reduction software	XENGEN ((HOWARD)
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000
High resolution limit [Å]	2.030	2.030 *
Rmerge	0.031	0.107 *
Total number of observations	23935 *
Number of reflections	7447	1033 *
Completeness [%]	90.0	65 *
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *		COMPND MOLECULE: MS2 UNASSEMBLED COAT PROTEIN DIMER. THIS BACTERIOPHAGE COAT PROTEIN WAS CRYSTALLIZED AS AN UNASSEMBLED DIMER; IT DID NOT FORM VIRAL CAPSIDS.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG8000	10 (%)
2	1	drop	protein	3 (mg/ml)
3	1	reservoir	citrate-phosphate	100 (mM)