1MSB
STRUCTURE OF THE CALCIUM-DEPENDENT LECTIN DOMAIN FROM A RAT MANNOSE-BINDING PROTEIN DETERMINED BY MAD PHASING
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.400, 73.100, 45.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.300 |
| Rwork | 0.176 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.600 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.031 * |
| Number of reflections | 7195 * |
| Completeness [%] | 89.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 1.1 (mM) | ||
| 5 | 1 | reservoir | PEG3350 | 15-20 (%) | or PEG8000 |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | 10 (mM) | ||
| 9 | 1 | reservoir | 0.02 (%) |
