1MRV
crystal structure of an inactive Akt2 kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2001-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 150.440, 150.440, 40.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.800 |
| Rwork | 0.247 |
| R-free | 0.30700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PKA in ternary complex |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.430 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.071 * |
| Total number of observations | 50158 * |
| Number of reflections | 11257 * |
| <I/σ(I)> | 8.7 |
| Completeness [%] | 94.4 * |
| Redundancy | 3.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | Li2SO4, PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 100 (mM) | pH8.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | beta-mercaptoethanol | 14 (mM) | |
| 6 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | PEG4000 | 15 (%) |
