1MR3

Saccharomyces cerevisiae ADP-ribosylation Factor 2 (ScArf2) complexed with GDP-3'P at 1.6A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	80
Detector technology	AREA DETECTOR
Collection date	1996-07-15
Detector	MARRESEARCH
Wavelength(s)	1.00805
Spacegroup name	P 21 21 2
Unit cell lengths	61.822, 69.470, 40.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.910 - 1.600
R-factor	0.204
Rwork	0.204
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hur
RMSD bond length	0.005
RMSD bond angle	1.200
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.910	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.045
Number of reflections	23034
<I/σ(I)>	22.9
Completeness [%]	96.2	90.7
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	30% PEG 8k, 100mM Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K