1MQJ
Crystal structure of the GluR2 ligand binding core (S1S2J) in complex with willardiine at 1.65 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.142, 93.223, 48.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.650 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.22900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mqi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.055 | 0.279 |
| Number of reflections | 35502 | |
| Completeness [%] | 99.2 | 93.7 |
| Redundancy | 5.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 4 * | PEG 5K MME, Na Citrate, zinc acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mM) | |
| 2 | 1 | reservoir | PEG5000 MME | 14-18 (%) | |
| 3 | 1 | reservoir | zinc acetate | 0.1 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.5 |
