1MPF
STRUCTURAL AND FUNCTIONAL ALTERATIONS OF A COLICIN RESISTANT MUTANT OF OMPF-PORIN FROM ESCHERICHIA COLI
Experimental procedure
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 117.900, 117.900, 52.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Number of reflections | 7337 |
| Completeness [%] | 84.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 9.8 * | Pauptit, R. A., (1991) J. Mol. Biol., 218, 505. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.05 (M) | |
| 3 | 1 | reservoir | n-octyl-2-hydroxyethylsulfoxide | 0.6 (%(w/w)) | |
| 4 | 1 | reservoir | octylPOE | 0.1 (%) | |
| 5 | 1 | reservoir | 0.7 (M) | ||
| 6 | 1 | reservoir | PEG2000 | 6 (%(w/w)) |
