1MOM
CRYSTAL STRUCTURE OF MOMORDIN, A TYPE I RIBOSOME INACTIVATING PROTEIN FROM THE SEEDS OF MOMORDICA CHARANTIA
Experimental procedure
Spacegroup name | H 3 |
Unit cell lengths | 131.170, 131.170, 40.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.160 |
R-factor | 0.186 |
RMSD bond length | 0.016 * |
RMSD bond angle | 0.047 * |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.160 * |
Rmerge | 0.100 * |
Number of reflections | 13591 * |
Completeness [%] | 96.9 * |
Redundancy | 4.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | phosphate | 20 (mM) | |
3 | 1 | reservoir | PEG4000 | 20-40 (%(w/v)) |