1MO0
Structural Genomics Of Caenorhabditis Elegans: Triose Phosphate Isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-22 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.399, 64.373, 105.706 |
| Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
| Resolution | 29.600 - 1.700 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.21300 |
| Structure solution method | SAS |
| Starting model (for MR) | THE STRUCTURE WAS SOLVED USING THE DATA COLLECTED ON R-AXIS IV WITH CU_KA RADIATION TO 2-A RESOLUTION. THE CRYSTAL WAS SOAKED WITH POTASSIUM IODIDE PRIOR DATA COLLECTION |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.000 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.039 * | 0.206 * |
| Total number of observations | 174695 * | |
| Number of reflections | 49994 * | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 92.7 | |
| Redundancy | 3.49 | 2.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 22 * | 2 M AMMONIUM SULFATE, 50 mM SODIUM ACETATE at pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.5 |
| 3 | 1 | drop | protein | 16 (mg/ml) | |
| 4 | 1 | drop | HEPES | 15 (mM) | pH7.5 |
