1MMP

MATRILYSIN COMPLEXED WITH CARBOXYLATE INHIBITOR

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-08-27
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	62.000, 62.000, 175.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.400 *
R-factor	0.18
Rwork	0.180
RMSD bond length	0.013
RMSD bond angle	2.760
Data reduction software	MOSFLM (V. 5.1.2)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.090
Total number of observations	80416 *
Number of reflections	15769
Completeness [%]	86.2
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	17-25 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	inhibitor	1.2 (mM)
3	1	drop	DMSO	2.5 (%)
4	1	reservoir	sodium citrate	0.2 (M)
5	1	reservoir	cacodylate	0.1 (M)
6	1	reservoir	2-propanol	30 (%)