1MM7
Crystal Structure of the GluR2 Ligand Binding Core (S1S2J) in Complex with Quisqualate in a Zinc Crystal Form at 1.65 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-03-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.912, 163.453, 47.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.650 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ftm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.445 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.051 | 0.197 |
| Number of reflections | 96906 | |
| Completeness [%] | 90.2 | 53.5 * |
| Redundancy | 5.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 | 4 * | PEG 8K, zinc acetate, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | quisqualate | 5 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 16-20 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.15-0.2 (M) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.5 |
