1MLA

THE ESCHERICHIA COLI MALONYL-COA:ACYL CARRIER PROTEIN TRANSACYLASE AT 1.5-ANGSTROMS RESOLUTION. CRYSTAL STRUCTURE OF A FATTY ACID SYNTHASE COMPONENT

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Detector technology	IMAGE PLATE
Collection date	1994-11-25
Detector	MARRESEARCH
Wavelength(s)	0.87
Spacegroup name	P 65
Unit cell lengths	68.180, 68.180, 118.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.184
Rwork	0.184
RMSD bond length	0.008
RMSD bond angle	0.033 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.500 *
Rmerge	0.059
Total number of observations	343991 *
Number of reflections	50284
Completeness [%]	98.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	0.01 (M)
3	1	drop	beta-octyl glucoside		0.6, 1.0 or 2.0 %(w/v)
4	1	reservoir	ammonium sulfate	45 (%sat)
5	1	reservoir	MES	0.1 (M)