1MKK
Disulfide deficient mutant of vascular endothelial growth factor A (C61A and C104A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-08-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8423 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.220, 75.470, 55.120 |
Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
Resolution | 28.000 - 1.320 |
R-factor | 0.16836 |
Rwork | 0.165 |
R-free | 0.19515 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vpf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 1.350 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmerge | 0.074 | 0.375 |
Number of reflections | 53321 | |
<I/σ(I)> | 12.08 | 2.48 |
Completeness [%] | 99.2 | 99.5 |
Redundancy | 3.8 | 3.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | isopropanol, PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-6 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | sodium citrate | 0.05 (M) | |
5 | 1 | drop | 2-propanol | 6 (%(v/v)) | |
6 | 1 | drop | PEG4000 | 14 (%) | |
7 | 1 | reservoir | sodium citrate | 0.1 (M) | |
8 | 1 | reservoir | 2-propanol | 12 (%(v/v)) | |
9 | 1 | reservoir | PEG4000 | 28 (%) |