1MJQ
METHIONINE REPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO AN ALTERED MET CONSENSUS OPERATOR SEQUENCE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 119.420, 119.420, 84.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.300 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26800 |
| Structure solution method | DIFFERENCE FOURIER METHODS |
| Starting model (for MR) | 1cma |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.860) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.170 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.046 | 0.197 * |
| Total number of observations | 125697 * | |
| Number of reflections | 51762 * | |
| <I/σ(I)> | 10.4 | 3.7 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 2.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | PROTEIN (10MG/ML) + SAM(1.5MG/ML) + DNA (4MG/ML) WAS CRYSTALLISED FROM 28-38% MPD, 100MM SODIUM CACODYLATE BUFFER, PH 6.0-7.0, 40MM CACL2 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PROTEIN | ||
| 2 | 1 | 1 | SAM | ||
| 3 | 1 | 1 | DNA | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | SODIUM CACODYLATE | ||
| 6 | 1 | 1 | CaCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | DNA | 4 (mg/ml) | |
| 3 | 1 | drop | SAM | 1.5 (mg/ml) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 8 | 1 | reservoir | MPD | 28-38 (%) |
