1MJL
METHIONINE REPRESSOR MUTANT (Q44K) COMPLEX WITH THE COREPRESSOR SAM (S-ADENOSYL METHIONINE) FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-03 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.790, 63.120, 43.970 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.19700 |
| Structure solution method | DIFFERENCE FOURIER METHODS |
| Starting model (for MR) | 1cmc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.700 * |
| Data reduction software | XDS |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.86) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | 0.049 |
| Number of reflections | 10414 | |
| <I/σ(I)> | 10.4 | 2.6 |
| Completeness [%] | 97.9 | 86.9 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | PROTEIN (10MG/ML) + SAM (1MG/ ML) WAS CRYSTALLIZED FROM 10-30% PEG 600, 100MM SODIUM CACODYLATE BUFFER, PH 4.6-5.2., pH 7.0 |
