1MJE
STRUCTURE OF A BRCA2-DSS1-SSDNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2001-10-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 198.866, 198.866, 200.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.500 |
| Rwork | 0.245 |
| R-free | 0.27830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.980 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 24950 | |
| Completeness [%] | 99.7 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | Sodium Acetate, Sodium Citrate, NaCl, DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Acetate | ||
| 2 | 1 | 1 | Sodium Citrate | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 2 | NaCl |
