1MJ2
METHIONINE REPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO A CONSENSUS OPERATOR SEQUENCE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.180, 117.180, 90.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.800 - 2.400 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26000 |
| Structure solution method | DIFFERENCE FOURIER METHODS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.860) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.046 | 0.222 * |
| Total number of observations | 103255 * | |
| Number of reflections | 27346 * | |
| <I/σ(I)> | 11.2 | 3.3 |
| Completeness [%] | 96.9 | 90 |
| Redundancy | 3.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | DNA | 4 (mg/ml) | |
| 3 | 1 | drop | SAM | 1.5 (mg/ml) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 8 | 1 | reservoir | MPD | 28-38 (%) |
