1MIZ

Crystal structure of an integrin beta3-talin chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-16
Detector	ADSC QUANTUM
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	37.533, 55.198, 46.751
Unit cell angles	90.00, 97.58, 90.00

Refinement procedure

Resolution	20.000 * - 1.900
R-factor	0.204
Rwork	0.204
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mix
RMSD bond length	0.006
RMSD bond angle	1.230 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP (CCP4)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.590	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.033 *	0.163 *
Total number of observations	36282 *
Number of reflections	13705
Completeness [%]	91.5 *	63.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	4 *	pH 7.0, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH7.0
2	1	reservoir	PEG2000 MME	20 (%(w/v))
3	1	drop	protein	10 (mg/ml)
4	1	drop	Tris-HCl	20 (mM)	pH7.0
5	1	drop		150 (mM)
6	1	drop	dithiothreitol	2 (mM)