1MHZ

METHANE MONOOXYGENASE HYDROXYLASE

Experimental procedure

Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1996-03-18
Detector	SIEMENS
Spacegroup name	C 2 2 21
Unit cell lengths	293.380, 64.010, 143.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.700
R-factor	0.152
Rwork	0.152
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mhy
RMSD bond length	0.013
RMSD bond angle	21.390 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	5.000
High resolution limit [Å]	2.700
Rmerge	0.094
Total number of observations	177040 *
Number of reflections	33478
<I/σ(I)>	6.18
Completeness [%]	89.3
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			used to seeding, Froland, W.A., (1994) J. Mol. Biol., 236, 379. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MMOH	2.5-3.5 (mg/ml)
2	1	drop	sodium phosphate/citrate	50 (mM)
3	1	drop	PEG2000	9-11 (%)
4	1	reservoir	PEG2000	18-22 (%)