1MFG
The Structure of ERBIN PDZ domain bound to the Carboxy-terminal tail of the ErbB2 Receptor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.605, 57.417, 30.439 |
| Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.250 |
| R-factor | 0.1297 |
| Rwork | 0.128 |
| R-free | 0.16400 * |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.01) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.025 * | 0.037 * |
| Number of reflections | 24313 | |
| Completeness [%] | 92.7 * | 91.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 * | 20 * | 12-15%PEG 4000, 100mM Ammonium Acetate, 100mM Sodium Acetate, 10% Glycerol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 19 (mg/ml) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | pH8.3 |
| 4 | 1 | reservoir | PEG4000 | 12-15 (%) | |
| 5 | 1 | reservoir | glycerol | 10 (%) | |
| 6 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 7 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
