1MEW

Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with XMP and NAD bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 4 3 2
Unit cell lengths	153.827, 153.827, 153.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.150
Rwork	0.224
R-free	0.24600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ak5
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.080	0.578 *
Total number of observations	734853 *
Number of reflections	33857
<I/σ(I)>	19	2.2
Completeness [%]	98.4	95.9
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	20 *	sodium malonate, Tris, 2-mercaptoethanol, EDTA, glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
10	1	reservoir	2-mercaptoethanol	1 (mM)
2	1	drop	Tris	50 (mM)	pH8.
3	1	drop		50 (mM)
4	1	drop	glycerol	10 (%(v/v))
5	1	drop	2-mercaptoethanol	1 (mM)
6	1	reservoir	ammonium sulfate	2.4 (M)
7	1	reservoir	Tris	100 (mM)	pH8.0
8	1	reservoir	glycerol	10 (%)
9	1	reservoir	PEG400	4 (mM)