1MDU
Crystal structure of the chicken actin trimer complexed with human gelsolin segment 1 (GS-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.187, 75.942, 96.747 |
| Unit cell angles | 90.00, 91.87, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.207 |
| Rwork | 0.193 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1esv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.164 |
| Number of reflections | 45313 | |
| <I/σ(I)> | 17 | 5.5 |
| Completeness [%] | 91.4 | 81.4 * |
| Redundancy | 4. * | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 4 * | PEG 3350, tris, calcium chloride, magnesium chloride, ADP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | PEG1000 | 15 (%(w/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 25 (mM) | pH8.0 |
| 4 | 1 | reservoir | ATP | 2 (mM) | |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | 2 (mM) |
