1MDM
INHIBITED FRAGMENT OF ETS-1 AND PAIRED DOMAIN OF PAX5 BOUND TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.270, 171.230, 44.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 * - 2.800 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1K78 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.360 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 * | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 * | 0.192 * |
| Number of reflections | 13405 | |
| <I/σ(I)> | 9.3 | 4 |
| Completeness [%] | 87.8 | 58.7 |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | 200mM Ammonium Acetate, 10% Peg4000, 100mM Acetate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | protein | 0.32 (mM) | |
| 4 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 5 | 1 | reservoir | acetate | 100 (mM) | pH5.0 |
| 6 | 1 | reservoir | PEG4000 | 10 (%) |
