1MDM

INHIBITED FRAGMENT OF ETS-1 AND PAIRED DOMAIN OF PAX5 BOUND TO DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-03-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	77.270, 171.230, 44.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 * - 2.800
R-factor	0.259
Rwork	0.259
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1K78
RMSD bond length	0.008 *
RMSD bond angle	1.360 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000 *	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.050 *	0.192 *
Number of reflections	13405
<I/σ(I)>	9.3	4
Completeness [%]	87.8	58.7
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	298	200mM Ammonium Acetate, 10% Peg4000, 100mM Acetate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	10 (mM)	pH8.0
2	1	drop	dithiothreitol	1 (mM)
3	1	drop	protein	0.32 (mM)
4	1	reservoir	ammonium acetate	200 (mM)
5	1	reservoir	acetate	100 (mM)	pH5.0
6	1	reservoir	PEG4000	10 (%)