1MD7
Monomeric structure of the zymogen of complement protease C1r
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2000-10-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 64 |
| Unit cell lengths | 167.060, 167.060, 43.648 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 3.200 |
| R-factor | 0.2428 |
| Rwork | 0.217 |
| R-free | 0.27860 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 25.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.120 * | 0.360 * |
| Number of reflections | 48443 * | |
| <I/σ(I)> | 5 | 1.8 |
| Completeness [%] | 95.9 * | 95.3 |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 293 | ammonium sulfate, TAPS, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | triethanolamine-hydrochloride | 50 (mM) | |
| 3 | 1 | drop | 145 (mM) | pH7.4 | |
| 4 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | reservoir | TAPS | 100 (mM) | pH8.4 |
